Toehold Switch Sensor Development Service
Biosensors have long been widely used in medicine, environmental safety, food safety, pathogen detection, and other fields for their sensitivity, portability, and ease of operation. Toehold switches are capable of recognizing a variety of specific RNA sequences and are used to detect small molecules, proteins, and nucleic acids. Nowadays, as scientists pay attention to toehold switches, toehold switches have been widely used in biosensor development. CD BioSciences, as a company that focuses on providing services related to toehold switches, mainly provides you with the following kinds of biosensor development services.
In this development service, CD BioSciences designs toehold switch RNAs based on target sequences and uses the designed toehold switch RNA to construct sensing plasmids. The sensing plasmid is loaded into an electrogenic circuit, and when the target is detected, the signal is output in the form of an electric current, which has the advantages of being label-free and easy to operate.
In this development service, CD BioSciences introduces dual-step pre-transduction based on traditional toehold switch RNA sensors. It simplifies the sensing process by converting target RNA into input signal through two consecutive toehold-mediated strand displacement reactions and can detect target RNA with highly folded or short sequences, which has the advantages of low cost, high-cost performance, and no complex operation such as probe labeling.
In this development service, CD BioSciences combines toehold switch RNA with paper carriers and cell-free systems, using NASBA isothermal amplification technology to amplify the target RAN and amplify the detection signal. The paper-based carrier is environmentally friendly and easy to carry. After our modification, it is equipped with a cell-free reaction system, which can provide you with a tailored paper-based cell-free toehold switch sensor with high sensitivity and high-cost performance.
In this development service, CD BioSciences uses reverse transcription loop-mediated amplification (RT-LAMP) instead of NASBA isothermal amplification technology to rapidly amplify target DNA. Then, under the action of RNA polymerase, the DNA amplicon is converted into RNA, which has participated in the cell-free reaction. We can develop a paper-based with RT-LAMP toehold switch sensor with higher sensitivity and shorter detection time for you.
In this development service, CD BioSciences introduces "Helper RNAs" in toehold switch-based biosensors. Applying helper RNAs around the target sequence will help us open up the mRNA secondary structure, making it easier to trigger the toehold switch, thereby increasing the sensitivity of the sensor to detect long mRNAs. We can provide you with a biosensor development service suitable for the detection of long mRNAs.
CD BioSciences provides several toehold switches-based biosensor development services with its advanced toehold switches technology platform and a research team with outstanding capabilities. If you are interested in our development services, please contact us directly.
For Research Use Only.