Development of Dual-Step Transduced Toehold Switch Sensor
Toehold switch has started to be used to develop biosensors due to its unique advantages. In the toehold switch sensor, specific target RNA sequences trigger the expression of reporter genes for biosensor detection purposes. The toehold switch sensor has the advantages of no labeling and easy portability, however, it also has some disadvantages, such as higher cost, the need to optimize the toehold switch according to different targets, the need to design repeat sequences, etc. In order to provide you with better toehold switch sensor development services, CD BioSciences is constantly optimizing the original services to meet your higher research requirements.
Our Service
Based on the toehold switch sensor, we introduce dual-step pre-transduction to provide you with a dual-step transduced toehold switch sensor development service. Dual-step pre-transduction can simplify the sensing process and avoid detection failure due to highly folded or too short target RNA sequences that cannot be turned on by toehold switch RNA. As with the traditional toehold switch sensor development service, in this service, we also need to design the toehold switch to connect to the reporter gene based on the specific target RNA sequence, except that we also need to add dual-step pre-transduction to the signal input part by two consecutive The toehold-mediated strand displacement reactions convert the target RNA into the input signal.
Our dual-step transduced toehold switch sensor development service has three main steps:
1. SD-transduction. This is the first step of two-signal transduction steps involving the recognition of a blocker (TP1 complex) of the target RNA. When the target RNA binds to the structural domain of the blocker, TP1 is released.
2. 4W-transduction, the second step of the two-signal transduction steps, involves the TP2 complex, and TP1 binds to the TP2 complex to form the 4W-associative trigger that turns on the toehold switch RNA.
3. Toehold switch reaction based on the 4W-associative trigger. 4W-associative trigger activates toehold switch RNA so that its hairpin structure is opened, ribosomes bind to ribosome binding sites, and linked reporter genes are translated.
Targets We Can Detect
- Bacteria
- Viruses
- Biomarkers
- Drug resistance genes, etc.
Our Advantages
- Low cost and high-cost performance
- Greatly simplifies the sensing process
- Increased sensitivity and accuracy
- Improved usability and portability of the device
- Eliminates the need for complex operations such as probe labeling
Applications
- Point-of-care testing
- Detection of RNA sequence diversity
- Development of tests for detection of viruses, intestinal microorganisms, etc.
We Are Ready to Provide
CD BioSciences provides you with a satisfactory dual-step transduced toehold switch sensor development service with our advanced technology platform. If you are interested in this service, please feel free to contact us, we are online 24 hours a day.
Reference
- Li, H.; et al. Homogeneous and Universal Detection of Various Targets with a Dual-Step Transduced Toehold Switch Sensor. Chembiochem. 2020, 21(10): 1418-1422.