Detection of Potato Virus Y Using Toehold Switches
Potato Y virus is a single-stranded positive-stranded ssRNA virus that causes tobacco vein spot virus disease and is transmitted mainly by aphids, but also by tubers. The Potato Y virus has a large number of hosts, is widely distributed, and occurs from time to time. Symptoms vary depending on the strain of the virus and include vein-banded mosaic, vein-spotted, and faded green spot types. Potato Y virus usually causes mild symptoms, but in severe cases it can lead to leaf deformation, terminal bud necrosis, and plant death, causing huge losses in potato and tobacco farming.
Conventional Testing Methods
The conventional detection methods of potato Y virus mainly include electron microscope observation method, serological detection method, identification method of sheath protein, and reverse transcription-polymerase chain reaction method. However, these methods have unavoidable disadvantages. Virus species cannot be identified using electron microscopy, and the equipment is expensive and requires a professional operation. Serological test results may be inaccurate in the early stages of potato Y virus infection. RT-PCR detection method has high specificity, but it has high cost and high requirements in the laboratory. To effectively detect and prevent the Potato Y virus, we need a low-cost, rapid, and site-agnostic detection method.
Toehold Switches in Potato Y Virus Detection
Toehold switches have received a lot of attention since they were discovered. Their ability to open their hairpin structure after being triggered by target RNA, allowing the translation of reporter genes to be translated, has enabled them to detect various pathogens. It has been shown that toehold switches when introduced as a sensor, can effectively detect potato Y virus and ensure food production. CD BioSciences, as an established toehold switches technology platform, can develop appropriate toehold switches-based biosensors for you and also provide you with toehold switches-based potato Y virus detection service.
The Development Services We Can Provide Based on Toehold Switches
The distribution of the potato Y virus, one of the viruses that severely damage food production, should be monitored. This requires the development of a site-independent detection device. At CD BioSciences, we have built a powerful toehold switches technology platform. With this technology platform, we can offer toehold switches-based biosensor development services for the potato Y virus for those involved in the development of potato Y virus detection tests. The main development service we are currently able to provide is as follows.
Our Testing Services Based on the Toehold Switches Platform
Potato virus Y is one of the most concerning pathogens in agriculture, and its detection is very important. CD BioSciences utilizes the high sensitivity and high specificity of toehold switches to provide potato Y virus detection service based on toehold switches for researchers engaged in potato Y virus-related research.
Advantages of Toehold Switches in Potato Y Virus Detection
- Low cost and high specificity.
- Enables rapid and immediate detection of the Potato Y virus.
- It can immediately control the spread of the virus and prevent the decline of food production.
CD BioSciences has long been committed to providing you with services related to toehold switches. We can provide you with customized sensor development services based on toehold switches to meet your specific pathogen detection needs. If you are interested in this technology, please feel free to contact us.
Reference
- Arce, A.; et al. Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts. Front Bioeng Biotechnol. 2021, 9: 727584.