Detection of Escherichia coli Using Toehold Switches
Escherichia coli (E. coli) is a facultative anaerobic gram-negative bacterium. E. coli is divided into different subtypes according to pathogenic, hemolytic, and enterotoxigenic types. Under normal circumstances, E. coli belongs to the normal flora in the intestinal tract of animals. Under special circumstances, it can cause gastrointestinal infections, urinary tract infections, and meningitis in humans and various animals. E. coli is mainly transmitted by the fecal-oral route. The infection is distributed worldwide, but it has certain regional characteristics and is related to the environment and hygiene. In order to control the spread of E. coli, it is not only necessary to improve the disease monitoring and reporting system, but also to develop new detection technologies with higher detection efficiency and higher sensitivity.
Conventional Testing Methods
The detection of E. coli usually adopts the traditional bacterial isolation method and uses selective culture to screen and identify it, which is complicated and time-consuming. Compared with traditional methods, PCR-based molecular detection technology can be more rapid, sensitive, simple, and efficient detection, but the detection limit is higher, and there will be false negatives caused by insufficient samples or false positives caused by contamination. Another gene chip technology is used in the detection of E. coli, but due to the high price of the instrument and strict technical operation, the application penetration rate of this technology is not high. The detection technology using biosensor technology has the advantages of fast analysis speed, online detection, and simple and easy operation. It becomes very important to develop such detection technology.
Toehold Switches in Escherichia coli Detection
Because of the editability and extensiveness of toehold switches in detecting target RNAs, some scholars have introduced E. coli-specific toehold switch RNAs as operating elements in the development of biosensors. At CD BioSciences, relying on high-quality toehold switches technology platform and a professional technical team, we can provide you with a comprehensive service for detecting E. coli using toehold switches.
The Development Services We Can Provide Based on Toehold Switches
CD BioSciences can provide toehold switches-related services to meet scientific research needs. With rich experience and advanced technology, we can provide you with a toehold switch-based biosensor development service for E. coli to meet your needs for rapid detection of E. coli in resource-poor environments.
Our Testing Services Based on the Toehold Switches Platform
The latest research reports show that toehold switches can be used to detect different pks gene islands of E. coli, and the detection performance can be further improved by (helper RNAs) 'Helper RNAs', which is conducive to further screening and stabilization of mRNA single-stranded regions. CD BioSciences relies on a strong technical R&D team, and the toehold switches platform built by CD BioSciences can provide professional detection services for R&D personnel and R&D teams engaged in E. coli-related target detection.
Advantages of Toehold Switches in Escherichia coli Detection
- Has a high degree of programmability and detection compatibility.
- It can efficiently complete the epidemic infection monitoring of E. coli.
- The detection of E. coli can be completed efficiently, accurately, and at a low cost.
CD BioSciences has been committed to the development of toehold switches-related technologies for a long time. We have a professional technical team, rich problem solutions, and provide a complete technology platform. If you need to develop E. coli detection equipment based on toehold switches, we can provide you with high-quality technical services. If you want to know more, please contact us directly.
Reference
- Heo, T.; et al. Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli. Life (Basel). 2021, 11(11): 1280.